Table 1

Forward and reverse primer sequences used for real-time PCR

Species

Gene

Forward sequence

Reverse sequence

Human

ADAMTS-4

5'-CCCCAGACCCCGAAGAGCCA-3'

5'-CCCGCTGCCAGGCACAGAAG-3'

Human

ADAMTS-5

5'-GGCCGTGGTGAAGGTGGTGG-3'

5'-GCTGCGTGGAGGCCATCGTC-3'

Human

ACAN

5'-ACCAGACGGGCCTCCCAGAC-3'

5'-TGGCTCTGCCCCAGAGGGAC-3'

Human

COL21A

5'-TGAGGGCGCGGTAGAGACCC-3'

5'-TGCACACAGCTGCCAGCCTC-3'

Human

IL-1Ra (pair 1)*

5'-CCCGTGAAGGAGAGCCCTTCATTTG-3'

5'-ACTTTCACCATCATTTCACAAATGCAG-3'

Human

IL-1Ra (pair 2)**

5'-TGTTCCATTCAGAGACGATCTGCCG-3'

5'-GAGCATGAGGCTCAATGGGTACC-3'

Human

MMP-13

5'-ATGCGGGGTTCCTGATGTGG-3'

5'-GGCCCAGGAGGAAAAGCATG-3'

Murine

Acan

5'-CAGTGCGATGCAGGCTGGCT-3'

5'-CCTCCGGCACTCGTTGGCTG-3'

Murine

Col2a1

5'-CACACTGGTAAGTGGGGCAAGACCG-3'

5'-GGATTGTGTTGTTTCAGGGTTCGGG-3'

Murine

IL-1Ra

5'-ACCCATGGCTTCAGAGGCAGC-3'

5'-GCCCCCGTGGATGCCCAAG-3'

*Primer pair used for IL-1Ra expression analysis in C28/I2 immortalized human chondrocytes and primary human chondrocytes for Figure 1A, 1C and Additional file 1. **Primer pair used for IL-1Ra expression analysis in primary human chondrocytes for Figure 4A and 4B. ACAN, aggrecan; COL2A1, type II collagen; IL-1Ra, interleukin-1 receptor antagonist; MMP-13, matrix metalloproteinase-13.

Jayasuriya et al. Arthritis Research & Therapy 2012 14:R197   doi:10.1186/ar4033