Table 1

Immunofluorescence patterns and titers of AIT test in SLE-CMV, SLE and CMV groups

Titer


Patterns from AIT test

≤1:20

1:40

1:80

1:160

1:320

1:640

1:1280

≥1:2560

Total


SLE-CMV groupa

Homogeneous

2

9

11

Homogeneous + speckled

4

13

17

Homogeneous + cytoplasmic

1

1

Homogeneous + diffuse granular

4

4

Homogeneous + speckled + cytoplasmic

3

3

Speckled

1

8

9

Speckled + cytoplasmic

1

1

2

Diffuse granular + cytoplasmic

1

1

Diffuse cytoplasmic + speckled

3

3

Diffuse cytoplasmic + diffuse granular

2

2

Total

0

0

0

1

0

6

1

45

53

SLE groupb

Homogeneous

1

2

15

18

Homogeneous + speckled

2

3

33

38

Homogeneous + cytoplasmic

2

2

Homogeneous + diffuse granular

1

12

13

Homogeneous + speckled + cytoplasmic

2

1

4

7

Speckled

5

3

5

34

47

Speckled + cytoplasmic

1

3

3

3

10

Diffuse granular + cytoplasmic

2

1

5

8

Diffuse granular

1

5

7

13

Diffuse cytoplasmic + speckled

9

9

Diffuse cytoplasmic + diffuse granular

1

1

2

Diffuse cytoplasmic + nucleolar

1

2

3

Total

0

0

0

0

12

10

22

126

170

CMV groupc

MTOC-MT

4

4

1

1

1

11

MTOC-MT + IF + speckled

1

1

2

1

5

Speckled

2

2

Negative

4

4

Total

4

1

7

6

1

1

2

0

22


aSLE-CMV group, anti-CMV IgM-positive SLE patients; bSLE group, anti-CMV IgM-negative SLE patients; cCMV group, anti-CMV IgM-positive patients without SLE. The AIT test was performed using an indirect immunofluorescent test kit (IT-AIT™; ImmunoThink Co., Seoul, Republic of Korea). In the CMV group, anti-MTOC-MT-positive patients accounted for 72.7% of the total patients in the CMV group and 88.9% of autoantibody-positive patients, but in the SLE-CMV group, anti-MTOC-MT was not detected at all (P < 0.001). Patients in the SLE-CMV group showed high titers of over 1:640 except for one who received steroid pulse therapy just before the test, but the proportion of patients in the CMV group with a titer over 1:640 was not more than 13.6% (P < 0.001). In addition, most of the autoantibody frequently found in the SLE-CMV group was not found in the CMV group. AIT, autoimmune target; CMV, cytomegalovirus; IF, intermediate filament; MTOC-MT, microtubule organizing center with microtubule; SLE, systemic lupus erythematosus.

Kim and Kim Arthritis Research & Therapy 2012 14:404   doi:10.1186/ar4023