Table 2

Primary and secondary antibodies for immunofluorescence staining

Primary antibodies

Secondary antibodies


Myofibroblasts

Rabbit anti-mouse ╬▒SMA Ab (Novus Biologicals, Littleton, CO); 1:100

Alexa fluor 488 donkey anti-rabbit IgG (Invitrogen, Grand Island, NY); 1:1,000


Fibrocytes

Rat anti-mouse CD45 Ab (BD Pharmingen, San Diego, CA); 1:50

Rabbit anti-mouse FSP1 Ab (Abcam, Cambridge, MA); 1:100

Alexa fluor 594 donkey anti-rat IgG (Invitrogen); 1:1,000

Alexa fluor 488 donkey anti-rabbit IgG (Invitrogen); 1:1,000


EndoMT

Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300

Rabbit anti-mouse FSP1 Ab (Abcam); 1:100

Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000

Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000


Stawski et al. Arthritis Research & Therapy 2012 14:R194   doi:10.1186/ar4028

Open Data