Open Access Research article

Fully recombinant IgG2a Fc multimers (stradomers) effectively treat collagen-induced arthritis and prevent idiopathic thrombocytopenic purpura in mice

Ajay Jain123, Henrik S Olsen4, Ravi Vyzasatya1, Erin Burch2, Yukimi Sakoda2, Emmanuel Y Mérigeon4, Ling Cai5, Changwan Lu5, Ming Tan5, Koji Tamada2, Dan Schulze26, David S Block4 and Scott E Strome26*

Author Affiliations

1 Division of General and Oncologic Surgery, University of Maryland School of Medicine, 22 South Greene Street, Room S4B12, Baltimore, MD 21201, USA

2 Department of Otorhinolaryngology-Head and Neck Surgery, University of Maryland School of Medicine, 16 S. Eutaw Street, Baltimore, MD 21201, USA

3 Baltimore Veterans Administration Medical Center, 10 N. Greene Street, 5C Surgical Services Area, Baltimore, MD 21201, USA

4 Gliknik Inc., 801 West Baltimore Street, Suite 501A, Baltimore, MD 21201, USA

5 Division of Biostatistics & Bioinformatics, Department of Epidemiology and Public Health & University of Maryland Greenebaum Cancer Center, University of Maryland School of Medicine, 660 W. Redwood Street, Suite 109, Baltimore, MD 21201, USA

6 Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 West Baltimore Street, Suite 385, Baltimore, MD 21201, USA

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Arthritis Research & Therapy 2012, 14:R192  doi:10.1186/ar4024

Published: 20 August 2012

Additional files

Additional file 1:

Figure S1, 2A-2HC multimer size correlates with binding stability to FcγRIIb and FcγRIII. This figure shows Octet biosensor assay data demonstrating that stradobody multimerization results in more stable associations with FcγRIIb and FcγRIIIa.

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Additional file 2:

Figure S2, 2A-2HC can cause an initial decrease in platelet count before platelet depletion with MWReg30 that may be the result of platelet sequestration by 2A-2HC. This figure demonstrates that 2A-2HC, in some experiments, caused an initial decrease in platelet count before administration of the platelet-depleting antibody MWReg30.

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Additional file 3:

Figure S3, 2A-2HC does not prevent graft-versus-host disease. This figure demonstrates that 2A-2HC cannot prevent T-cell-mediated graft-versus-host disease in a murine model.

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Additional file 4:

Figure S4, The FcγRIIb-/- and "wild type" C57/BL6 have differing baseline platelet and red blood cell counts. This figure demonstrates that FcγRIIb-/- and "wild type" C57/BL6 have differing baseline platelet counts, which could potentially affect the dynamic range of ITP assay in FcγRIIb-/- mice.

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