Table 1

Summary of mAb specificities

Clones

Mice

Isotypes

Rat COMP

Mouse COMP

Human COMP

Binding sites


1A12

COMP-/-

IgG1

++

++

_

EGF-like domain

1B8

COMP-/-

IgG1

+

+

_

TSP3 domain

1D10

COMP-/-

IgG1

++

++

++

C-terminal globular domain

2D3

COMP-/-

IgG1

+++

+++

+++

coiled-coil domain or pentameric COMP

1C1

COMP-/-

IgG2b

+

++

_

EGF-like domain

3C7

COMP-/-

IgG2b

+

_

_

ND

3F8

COMP-/-

IgG2b

++

++

_

EGF-like domain

4A8

COMP-/-

IgG2b

++

++

_

C-terminal globular domain

8E3

COMP-/-

IgG1

++

+

_

EGF-like domain

12H8

COMP+/+

IgG1

+++

+++

_

EGF-like domain

15A11

COMP+/+

IgG1

+++

+++

+++

EGF-like domain

4D12

COMP-/-

IgG2b

+

_

_

EGF-like domain

3F11

COMP-/-

IgG2a

++

+

_

EGF-like domain

16B5

COMP+/+

IgG1

+++

+++

+++

coiled-coil domain or pentamer COMP

18E7

COMP-/-

IgG2b

+

+

_

EGF-like domain

5E8

COMP-/-

IgG2b

+

_

_

ND

6H4

COMP-/-

IgG2b

++

++

+

EGF-like domain

29C3

COMP-/-

IgG1

+

+++

_

EGF-like domain

30G5

COMP-/-

IgG2b

++

++

_

C-terminal globular domain

23A8

COMP-/-

IgG1

++

+++

_

EGF-like domain

24F7

COMP-/-

IgG2b

++

++

_

EGF-like domain


Grading of values obtained with mAbs using culture supernatants. Scale for optical density (OD) values: -, < 3 times standard error of the mean (SEM) of negative control; +, > 3 times SEM of negative control; ++, > 0.5 OD; +++, > 1.0 OD; COMP, cartilage oligomeric matrix protein; EGF, epithelial growth factor; ND, not determined.

Geng et al. Arthritis Research & Therapy 2012 14:R191   doi:10.1186/ar4022

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