Figure 5.

Immunohistochemical analysis of cartilage oligomeric matrix protein (COMP)-specific mAbs binding to cartilage in vitro. Immunohistochemistry was performed using 7 μm cryo-sections from naive neonatal mouse limbs, using 5 μg/ml of biotinylated COMP-specific mAbs. Binding was detected by extravidin-peroxidase and DAB substrate. Sections from COMP^+/+ mouse limbs stained with 12H8, 15A11, 1D10, 3F8, 1B8, 16B5 are depicted in the pictures. Sections from COMP^-/- mouse limbs stained with 12H8, 15A11, 1D10, 3F8, 1B8, 16B5 were used as negative controls. Anti-collagen type II (CII) monoclonal M2139 served as the positive control. The positive monoclonal staining on cartilage and tendon is indicated with an arrow.