Epitope mapping of 4 representative COMP-specific mAbs by using full-length cartilage oligomeric matrix protein (COMP) and recombinant fragments. B cell hybridomas were produced from COMP+/+Ncf1*/* or COMP-/-Ncf1*/* immunized with rat COMP. Monoclonal antibodies were obtained from culture supernatants after 14 days and tested by ELISA. Plates were individually coated with 10 μg/ml of recombinant full-length mouse COMP or COMP fragments. Serum samples from naïve mice served as negative controls and serum samples from COMP immunized mice were used as positive controls.
Geng et al. Arthritis Research & Therapy 2012 14:R191 doi:10.1186/ar4022