Figure 2.

Epitope mapping of 4 representative COMP-specific mAbs by using full-length cartilage oligomeric matrix protein (COMP) and recombinant fragments. B cell hybridomas were produced from COMP^+/+Ncf1^*/* or COMP^-/-Ncf1^*/* immunized with rat COMP. Monoclonal antibodies were obtained from culture supernatants after 14 days and tested by ELISA. Plates were individually coated with 10 μg/ml of recombinant full-length mouse COMP or COMP fragments. Serum samples from naïve mice served as negative controls and serum samples from COMP immunized mice were used as positive controls.