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Open Access Highly Accessed Research article

Atorvastatin inhibits osteoclastogenesis by decreasing the expression of RANKL in the synoviocytes of rheumatoid arthritis

Jeong Yeon Kim1, Eun Young Lee1, Eun Bong Lee1, Yun Jong Lee2, Hyun Jung Yoo1, Jiyong Choi1 and Yeong Wook Song13*

  • * Corresponding author: Yeong W Song ysong@snu.ac.kr

  • † Equal contributors

Author Affiliations

1 Division of Rheumatology, Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 110-744, Korea

2 Division of Rheumatology, Department of Internal Medicine, Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-707, Korea

3 WCU Department of Molecular Medicine and Biopharmaceutical Sciences, Medical Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul, 110-799, Korea

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Arthritis Research & Therapy 2012, 14:R187  doi:10.1186/ar4018

Published: 17 August 2012

Additional files

Additional file 1:

Figure S1. Effects of simvastatin on receptor activator of nuclear factor κB ligand (RANKL) expression in fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA) patients (patients 1, 2, and 3). FLSs were isolated from three RA patients and cultured in the presence of TNF-α (20 ng/ml) with or without simvastatin (10 to 100 μM) for 24 hours. RANKL expressions were analyzed with RT-PCR (A) and Western blotting (B).

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Additional file 2:

Figure S2. Effects of mevalonate on the statin-induced suppression of RANKL. FLSs from RA patients (patients 1, 2, and 3) were cultured in the presence of TNF-α (20 ng/ml), and then simvastatin (50 to 100 μM) and mevalonate (100 μM) were added for 24 hours. RANKL expressions were analyzed with RT-PCR (A) and Western blotting (B).

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Additional file 3:

Figure S3. The effects of simvastatin on cell viability and apoptosis. (A) Effects of simvastatin on the viability of FLSs from an RA patient (patients 1, 2, and 3). Cells were cultured for 24 hours in the presence of TNF-α (20 ng/ml) and simvastatin at different concentrations (10 to 100 μM). Cell viabilities were determined by using MTT assays. (B) Effects of simvastatin on the apoptosis of FLSs, as determined by propidium iodide staining.

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