Binding of autoantibodies to FH fragments. Molar equivalent concentrations of the FH fragments (corresponding to CCPs 8 to 20, CCPs 15 to 20, CCPs 19 to 20), FHL-1 (CCPs 1 to 7), FH and BSA (negative control) were coated onto ELISA plates and autoantibody binding was determined with rabbit anti-human IgG antibodies followed by swine anti-rabbit HRP antibodies. The FH fragments used are schematically presented in panel A. Results are shown as mean values of three independent experiments +/- SD.
Foltyn Zadura et al. Arthritis Research & Therapy 2012 14:R185 doi:10.1186/ar4016