ELISA analysis of urine and serum transferrin levels. Transferrin levels in urine of healthy controls (N = 10), inactive SLE (N = 10) and active SLE (N = 10) patients were measured by ELISA (Genway Biotech, San Diego, CA, USA). Transferrin expression was increased by 87-fold (P = 0.002) in active SLE patients compared with healthy controls, and by 14-fold (P = 0.003) when compared with inactive SLE patients. Urine transferrin/UCr levels correlated significantly with urine ferritin/Cr levels (R = 0.45, P = 0.005), disease activity, (R = 0.41, P = 0.033), and SLEDAI (R = 0.418, P = 0.033). Data represent mean ± SEM.
Vanarsa et al. Arthritis Research & Therapy 2012 14:R182 doi:10.1186/ar4012