Figure 6.

Incubation of monocytes under hypoxia leads to translocation of transcription factor, nuclear factor of kappa light polypeptide gene enhancer in B-cells (NFκB1), into the nucleus. Detection of hypoxia-inducible factor alpha (HIF-1α), Lamin B and β-actin in nuclear (NF+) and cytosolic (NF-) cell fractions of primary human monocytes using immunoblot. (A-C) Monocyte protein lysates were acquired after incubation for 5 h under hypoxia and under normoxia as indicated. The cellular localization of transcription factors NFκB p100/p52, c-Rel, and c-Jun (Figure 6A), NFκBp105/p50 and c-Fos (Figure 6B), and Jun B and NFκB p65 (Figure 6C) were determined by immunoblot. NFκBp105 (the inactive form of NFκB1) remains in the cytoplasm, while the active form NFκBp50 is translocated into the nucleus under hypoxic conditions (Figure 6B). Other factors showed either no change in their localization or, as for c-Jun and NFκBp52, were no longer translocated into the nucleus under hypoxic incubation.