Monocytes, in contrast to human monocyte derived macrophages (hMDMs), show hypoxia-induced gene expression. Real-time PCR analysis of mRNA expression levels of the glycolytic enzymes LDHA (A) and PGK1 (B), the hypoxia inducible factor (HIF)-1 target gene CXCR4 (C) and the transcription factor HIF1A (D) in monocytes and hMDM that were incubated under normoxia (white bars) and under hypoxia (black bars), respectively (n = 6). The mRNA expression level of ß-actin (ACTB) was used for normalization. ΔCt = Ct(GOI) - Ct(ACTB). Values are means ± SD. +P < 0.1;* P < 0.05; (Wilcoxon t-test).
Fangradt et al. Arthritis Research & Therapy 2012 14:R181 doi:10.1186/ar4011