Table 1 |
||||||
|
Primer sequences used for real-time reverse transcription-polymerase chain reaction |
||||||
|
Primer |
Accession number |
Direction (5' → 3') |
Sequence |
Melting temperature, °C |
Product length, base pairs |
Reference |
|
|
||||||
|
ADAMTS-4 |
NM_181667.1 |
Forward |
AACTCGAAGCAATGCACTGGT |
54.96 |
149 |
[40] |
|
Reverse |
TGCCCGAAGCCATTGTCTA |
53.16 |
||||
|
GAPDH |
NM_001034034.1 |
Forward |
ATCAAGAAGGTGGTGAAGCAGG |
54.61 |
101 |
[40] |
|
Reverse |
TGAGTGTCGCTGTTGAAGTCG |
55.17 |
||||
|
INOS |
NM_001076799.1 |
Forward |
GTAACAAAGGAGATAGAAACAACAGG |
52.30 |
146 |
[16] |
|
Reverse |
CAGCTCCGGGCGTCAAAG |
55.52 |
||||
|
IL-1β |
NM_174093.1 |
Forward |
GAGCCTGTCATCTTCGAAACG |
59.34 |
55 |
This paper |
|
Reverse |
GCACGGGTGCGTCACA |
60.32 |
||||
|
IL-6 |
NM_173923.2 |
Forward |
GCTTCACAAGCGCCTTCACTCC |
64.47 |
81 |
This paper |
|
Reverse |
GACCCGGGGTAGGGAAAGCAGA |
65.73 |
||||
|
MMP-13 |
NM_174389.2 |
Forward |
TGGTCCAGGAGATGAAGACC |
52.51 |
80 |
[40] |
|
Reverse |
TGGCATCAAGGGATAAGGAA |
50.22 |
||||
|
TLR-4 |
NM_174198.6 |
Forward |
TGCGTACAGGTTGTTCCTAACATT |
54.79 |
110 |
This paper |
|
Reverse |
TAGTTAAAGCTCAGGTCCAGCATCT |
55.50 |
||||
|
|
||||||
|
ADAMTS-4, a disintegrin and metalloproteinase with thrombospondin motifs 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-1β, interleukin-1 beta; IL-6, interleukin-6; INOS, inducible nitric oxide synthase; MMP-13, matrix metalloproteinase 13; TLR-4, Toll-like receptor 4. |
||||||
|
Gorth et al. Arthritis Research & Therapy 2012 14:R179 doi:10.1186/ar3932 |
||||||
