Table 1

The primer sequences for real-time PCR.

gene

Forward and Reverse Primers

ALP

5'-TGGACCTCGTTGACACC-3' 5'-TCCTGTTCAGCTCGTACT -3'

BSP

5'-ATACAGGGTTAGCTGCAATC-3'

5'- TCCATTGTCTCCTCCGC -3'

OCN

5'-GGCGCTACCTGTATCAATG-3' 5'-AGAGCGACACCCTAGAC -3'

OSX

5'-CCACCTACCCATCTGACT-3' 5'-GTTTGGCTCCACCACTC -3'

Runx2

5'-TATTGGTCCTAAGGGAGACATC-3' 5'-TAAAGCGAATGGGCATGTT -3'

CA1

5'-GCTACAGGCTCTTTCAGTT-3' 5'-GACTCCATCCACTGTATGTT -3'

GAPDH

5'-ACCACAGTCCATGCCATCAC-3' 5'-TCCACCACCCTGTTGCTGTA -3'

Chang et al. Arthritis Research & Therapy 2012 14:R176   doi:10.1186/ar3929