Measuring cell proliferation by the MTT assay. Saos-2 cells were incubated in (1) normal culture medium, (2) osteogenic medium, (3) osteogenic medium supplemented with DMSO, and (4) osteogenic medium supplemented with 10 μM, 100 μM and 1 mM of acetazolamide (AZ). The results of the MTT assay indicated that the cell proliferation was not significantly affected by osteogenic medium, DMSO or 100 μM AZ. This experiment was repeated three times and similar results were obtained. The error bars represent the standard deviation from five replicate measurements. DMSO, dimethyl sulfoxide; MTT, 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide; OM, osteogenic medium.
Chang et al. Arthritis Research & Therapy 2012 14:R176 doi:10.1186/ar3929