Figure 3.

Receiver-operating characteristic (ROC) analysis of the absolute numbers of circulating monocytes and their subsets. Receiver-operating characteristic (ROC) analysis of the absolute numbers of circulating monocytes, and their CD14^+highCD16^-, CD14^+highCD16⁺ and CD14^+lowCD16⁺ subsets, at three months of anti-TNFα treatment (A, B, C and D, respectively). The predictive value of the absolute numbers of monocytes was determined by calculating the area under the curve (AUC). The optimum cut-offs (cells/μl) to distinguish MTX responders from non-responders, plus their sensitivity (Sens), specificity (Spec), positive predictive value (PPV), negative predictive value (NPV) and likelihood ratio (LR), are illustrated next to the curves. These were used to verify the validation of the ROC curves and to establish the predictive power of the cut-offs.