Additional file 2.

Figure S2. Relative expression of IL-27 p28 mRNA in liver. To compare the transgene expression, livers in each group were collected at the end of experiments (20-week of post delivery periods) and total RNAs were extracted for cDNA synthesis. PCR primers were designed using IDT's PrimerQuestSM (Integrated DNA Technologies Inc., Coralville, IA, USA). Quantitative realtime PCR was performed using the iCycler IQTM multi-color realtime PCR detection system (Bio-Rad Laboratories). The Ct values obtained were normalized to those of 18S ribosomal RNA. Level of IL-27 p28 mRNA in LacZ or IL-27 delivered group were normalized against the mRNA level in saline group. (values are mean ± SEM, *p < 0.05 rAAV2-IL27 group versus rAAV2-LacZ or saline groups by one-way ANOVA test).

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Lee et al. Arthritis Research & Therapy 2012 14:R172   doi:10.1186/ar3925