Nuclear speckled anti-nuclear antibody staining pattern profile. (A) Representative images of nuclear speckled anti-nuclear antibody at a dilution of 1:400. Sera were collected in the pre-disease group at 6 weeks of age (before treatment) and 26 weeks of age or 20 weeks after treatment. In the clinical disease group, sera were collected at 14 weeks of age (before treatment) and 34 weeks of age or 20 weeks after treatment. Images were captured at ×200 magnification with a Zeiss Axiovert 200 M microscope with a constant exposure of 0.3 seconds. (B) Nuclear speckled titers in pre-disease treated mice. (C) Nuclear speckled titers in clinical disease treated mice. Three individuals in blinded fashion determined autoantibody titers. Values are mean ± standard error of the mean (n = 7). *P < 0.05 rAAV2-IL27 group versus rAAV2-LacZ or saline groups by one-way analysis-of-variance test. IL-27, rAAV2-IL27-treated groups; LacZ, rAAV2-LacZ-treated groups; Saline, saline-treated groups. These symbols have no specific meanings because the legends on the x-axis defined them. However, if we must define these symbols, are you ok with? Pre-disease stage: 6 weeks old mice (inverted triangle), saline-treated mice (circle), rAAV2-LacZ-treated mice (square), rAAV2-IL27-treated mice (triangle). Clinical-disease stage: 14 weeks old mice (inverted triangle), saline-treated mice (circle), rAAV2-LacZ-treated mice (square), rAAV2-IL27-treated mice (triangle).
Lee et al. Arthritis Research & Therapy 2012 14:R172 doi:10.1186/ar3925