rAAV2-IL27-expressing vector is capable of negatively regulating splenic TH17 cells. Splenocytes were isolated from each mouse and stained for CD3 and CD4 fluorochrome-conjugated antibodies. Cells were further intracellularly stained for IL-27, IL-17, and IFN-γ. Flow cytometry analysis was performed with cells gated on CD4+ populations. Values are mean ± standard error of the mean (n = 7). *P < 0.05 rAAV2-IL27 group versus rAAV2-LacZ or saline groups by one-way analysis-of-variance test; **P < 0.01. IFN-γ, interferon-gamma; IL, interleukin; IL-27, rAAV2-IL27-treated groups; LacZ, rAAV2-LacZ-treated groups; Saline, saline-treated groups.
Lee et al. Arthritis Research & Therapy 2012 14:R172 doi:10.1186/ar3925