Generation of functional IL-27-expressing vector (rAAV2-IL27). (A) IL-27 p28 expression levels in the cell lysates or culture media of mock transfection which contain transfection reagents only (white bar) or control empty AAV2 plasmid (grey bar) or rAAV2-IL27 (black bar) transfected HEK293 cells at 48 hours after transfection. (B) Biological effects of IL-27 were generated from rAAV2-IL27 on C57BL/6 splenocytes by using media only or (a) mock, (b) IL-17 differentiation cocktail containing IL-6 and transforming growth factor-beta, or (c) IL-17 differentiation cocktail with supernatant IL-27 from rAAV2-IL27 transfected HEK293 cells (sIL-27). The experiment was done in triplicate and repeated three times for consistency. Values in bar graphs are mean. **P < 0.01, rAAV2-IL27 group versus mock or rAAV2 transfected by one-way analysis-of-variance test. IFN-γ, interferon-gamma; IL, interleukin.
Lee et al. Arthritis Research & Therapy 2012 14:R172 doi:10.1186/ar3925