Table 2

Angiogenesis-related genes altered during the course of acute CIA

Gene

Gene bank

Gene name

Onset

Day 4

Day 8

Day 12


Fold change

P-value

Fold change

P-value

Fold change

P-value

Fold change

P-value


Growth factors and receptors

Ang-1

NM_009640

Angiopoietin 1

1.3

1.8

2.7

0,004

1.5

Edg1

NM_007901

Endothelial differentiation sphingolipid G-protein-coupled receptor 1

1.8

2.4

0,001

3.3

0,002

2.4

0,005

Egf

NM_010113

Epidermal growth factor

-1.4

-2.9

0,013

-3.7

0,005

-2.9

0,005

Ereg

NM_007950

Epiregulin

-1.4

-2.1

0,001

-2.3

0,002

-1.8

Fgf-1

NM_010197

Fibroblast growth factor 1

-1.3

-2.3

0,004

-1.7

-1.1

Flk-1

NM_010612

Fetal liver kinase

1.3

2.2

0,011

3.0

0,006

1.9

Hgf

NM_010427

Hepatocyte growth factor

2.2

0,006

4.6

0,001

6.0

0,001

3.9

0,002

Igf-1

NM_010512

Insulin-like growth factor 1

1.4

2.4

0,000

4.1

0,001

2.4

0,000

Lep

NM_008493

Leptin

-2.5

0,007

-5.8

0,002

-6.8

0,002

-3.9

0,015

Mdk

NM_010784

Midkine

1.5

2.4

0,008

7.5

0,002

5.3

0,003

Nrp-2

NM_010939

Neuropilin 2

1.4

2.0

0,009

2.4

0,015

2.2

0,011

Tgfβ1

NM_011577

Transforming growth factor, beta 1

1.6

2.1

0,009

2.3

0,005

1.8

Tgfβ2

NM_009367

Transforming growth factor, beta 2

-1.4

-2.0

0,021

-1.3

1.1

Tgfβr1

NM_009370

Transforming growth factor, beta receptor I

1.5

1.6

2.0

0,003

1.5

Proteases and inhibitors

Mmp-2

NM_008610

Matrix metallopeptidase 2

1.7

2.6

0,001

4.1

0,000

3.0

0,001

Mmp-9

NM_013599

Matrix metallopeptidase 9

3.4

0,002

16.2

0,001

20.2

0,001

12.7

0,001

Mmp-19

NM_021412

Matrix metallopeptidase 19

3.6

0,001

4.6

0,002

5.2

0,000

3.8

0,003

Timp-1

NM_011593

Tissue inhibitor of metalloproteinase 1

17.1

0,009

34.0

0,001

24.4

0,001

11.6

0,001

Timp-2

NM_011594

Tissue inhibitor of metalloproteinase 2

1.2

1.4

2.4

0,001

2.1

0,001

Adhesion molecules

Cdh5

NM_009868

Cadherin 5

1.6

3.0

0,003

3.5

0,002

2.3

0,017

Eng

NM_007932

Endoglin

1.6

1.9

2.6

0,007

1.9

Itgβ3

NM_016780

Integrin beta 3

1.9

5.9

0,001

6.1

0,001

3.4

0,001

Pecam1

NM_008816

Platelet/endothelial cell adhesion molecule 1

1.7

1.7

2.1

0,012

1.8

Stab1

NM_138672

Stabilin 1

2.6

0,000

4.0

0,001

3.3

0,001

2.0

0,002

Transcription factors

Efnβ2

NM_010111

Ephrin B2

-2.0

-2.5

0,001

-1.7

-1.1

Hif-1α

NM_010431

Hypoxia inducible factor 1, alpha subunit

1.5

2.6

0,001

3.8

0,000

1.6

Tbx1

NM_011532

T-box 1

-1.7

-3.3

0,003

-3.3

0,011

-2.0

0,013

Tnfαip2

NM_009396

Tumor necrosis factor, alpha-induced protein 2

2.0

0,002

2.6

0,004

2.6

0,001

2.0

0,001

Anti-angiogenic factors

Tsp-1

NM_011580

Thrombospondin 1

1.8

1.9

2.8

0,000

2.0

0,006

Tsp-2

NM_011581

Thrombospondin 2

1.3

2.2

0,003

2.9

0,001

2.0

0,002

Col4α3

NM_009929

Procollagen, type IV, alpha 3/tumstatin

1

-2.0

0,015

-2.0

0,011

-1.3

Col18α1

NM_007734

Procollagen, type XVIII, alpha 1/endostatin

1.1

1.7

2.0

0,012

2.0

0,015

Cytokinesand chemokines

Il-1β

NM_008361

Interleukin 1 beta

34.1

0,000

48.2

0,000

60.0

0,000

16.2

0,000

Il-6

NM_031168

Interleukin 6

183.7

0,000

190.4

0,000

164.7

0,000

30.3

0,001

Tnfα

NM_013693

Tumor necrosis factor

2.2

0,014

2.2

0,009

3.7

0,010

2.2

0,018

Ccl2

NM_011333

Chemokine (C-C motif) ligand 2/MCP1/HC11

15.0

0,000

11.0

0,001

10.0

0,000

3.0

0,001

Cxcl1

NM_008176

Chemokine (C-X-C motif) ligand 1/Gro1

19.2

0,003

35.7

0,001

21.0

0,001

7.0

0,019

Cxcl2

NM_009140

Chemokine (C-X-C motif) ligand 2/Grob

8.7

0,001

13.4

0,001

15.2

0,001

6.1

0,001

Cxcl5

NM_009141

Chemokine (C-X-C motif) ligand 5/ENA-78

126.3

0,002

378.4

0,001

345.8

0,001

70.3

0,003

Ccl11

NM_011330

Small chemokine (C-C motif) ligand 11/eotaxin

1.1

-2.0

0,003

-2.0

0,019

1.2

Csf3

NM_009971

Colony stimulating factor 3 (granulocyte)/G-CSF

86.9

0,016

69.7

0,017

48.9

0,020

20.3

0,034


Angiogenesis RT2-Profiler PCR arrays (APM-024; SuperArray Bioscience, Frederick, MD) were performed using cDNA from paws of CIA mice at different time points post-arthritis onset. For each time point, RNA from four animals was pooled and analysed in duplicate. Data are expressed as fold change relative to the expression in paws from healthy mice after normalisation to four housekeeping genes. The table lists genes that exhibit at least a two-fold difference in expression at one or more time-points in arthritic tissue when compared to control tissue. Data were analysed by paired t-test.

Raatz et al. Arthritis Research & Therapy 2012 14:R169   doi:10.1186/ar3922

Open Data