Blocking neutropilin (NRP)-1 inhibits human microvascular endothelial cells (HMEC)-1 migration induced by arthritic paw homogenates. The ability of anti-NRP1B antibody to inhibit the migration of HMEC-1 in response to paw homogenates was evaluated using a chemotaxis assay. Cells were seeded in presence of anti-NRP1B (10 µg/ml) or PBS into the upper chamber and allowed to migrate towards paw homogenates (100 µg protein/ml) in the lower chamber. PBS as stimulant was used as a negative control (set as 1, dashed line). Data expressed as mean ± SEM (healthy, n = 3; arthritic, n = 6). Data were analysed using two-way ANOVA: ***P < 0.001.
Raatz et al. Arthritis Research & Therapy 2012 14:R169 doi:10.1186/ar3922