Table 1 |
||||
|
Primer details used for RT-PCR analyses |
||||
|
Gene |
RT-PCR primer sequences (5' to 3') |
Annealing temperature (°C) |
Product size (base pairs) |
Cycles |
|
|
||||
|
Chondrogenic markers |
||||
|
COL type II |
Sense: TTTCCCAGGTCAAGATGGTC |
58.0 |
374 |
35 |
|
Antisense: CTTCAGCACCTGTC CACCA |
||||
|
AGN |
Sense: TGAGGAGGGCTGGAACAAGTACC |
54.0 |
392 |
30 |
|
Antisense: GGAGGTGGTAATTGCAGGGAACA |
||||
|
COMP |
Sense: CAGGACGACTTTGATGCAGA |
54.0 |
312 |
32 |
|
Antisense: AAGCTGGAGCTGTCTGGTA |
||||
|
FMD |
Sense: CTTACCCCTATGGGGTGGAT |
54.0 |
389 |
35 |
|
Antisense: GTACATGGCCGTGAGGAAGT |
||||
|
SOX9 |
Sense: ATCTGAAGAAGGAGAGCGAG |
58.0 |
263 |
35 |
|
Antisense: TCAGAAGTCTCCAGAGCTTG |
||||
|
Hypertrophy and osteogenic markers |
||||
|
COL type X |
Sense: CCCTTTTTGCTGCTAGTATCC |
54.0 |
468 |
25 |
|
Antisense: CTGTTGTCCAGGTTTTCCTGGCAC |
||||
|
OP |
Sense: ACGCCGACCAAGGAAAACTC |
51.0 |
483 |
35 |
|
Antisense: GTCCATAAACCACACTATCACCTCG |
||||
|
COL type I |
Sense: GGACACAATGGATTGCAAGG |
54.0 |
461 |
32 |
|
Antisense: TAACCACTGCTCCACTCTGG |
||||
|
OC |
Sense: ATGAGAGCCCTCACACTCCTC |
59.0 |
387 |
35 |
|
Antisense: GCCGTAGAAGCGCCGATAGGC |
||||
|
ALP |
Sense: TGGAGCTTCAGAAGCTCAACACCA |
51.0 |
454 |
35 |
|
Antisense: TCTCGTTGTCTGAGTACCAGTCC |
||||
|
Internal control |
||||
|
EF-1α |
Sense: AGGTGATTATCCTGAACCATCC |
54.0 |
234 |
25 |
|
Antisense: AAAGGTGGATAGTCTGAGAAGC |
||||
|
|
||||
|
AGN, aggrecan core protein; ALP, alkaline phosphatase; COL, collagen; COMP, cartilage oligomeric matrix protein; EF-1α, elongation factor 1α; FMD, fibromodulin; OC, osteocalcin; OP, osteopontin; SOX9, sex determining region Y-box 9. |
||||
|
Steinert et al. Arthritis Research & Therapy 2012 14:R168 doi:10.1186/ar3921 |
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