Figure 2.

Transgene expression of mesenchymal stem cell aggregates following adenoviral Indian hedgehog gene transfer. Primary mesenchymal stem cells were modified with Ad.GFP (controls), Ad.IHH alone (IHH), or in combination with Ad.TGF-β1 (IHH + TGF-β1) or Ad.BMP-2 (IHH + BMP-2), seeded into pellets and analysed during a 21-day time course. Values represent levels of (a) IHH, (b) TGF-β1, or (c) BMP-2 transgene product in the conditioned media by the respective groups over 24 hours at the time points indicated. Mean ± standard deviation from three aggregates per condition and marrow preparation from three marrow preparations from different patients is shown. *Significant differences (P < 0.05) compared with controls. Ad., adenoviral vector; BMP, bone morphogenetic protein; IHH, Indian hedgehog; TGF-β, transforming growth factor beta.