Table 1

Bone marrow stromal cells from all three mouse strains had characteristics of mesenchymal stem cells in terms of morphology, differentiation capacity and cell surface markers.

Mouse Strain

DBA/1

FVB

BALB/c


MHC H2 Haplotype

H2q

H2q

H2d

Morphology

Fibroblastic

Fibroblastic

Fibroblastic

Adipogenesis

Positive

Positive

Positive

Osteogenesisa

(μg Ca/well)

6.9 ± 1.6

48.0 ± 0.9

11.9 ± 0.2

Chondrogenesisa

(μg GAG/pellet)

5.6 ± 0.3

5.6 ± 0.5

5.6 ± 0.3

CD73b

64.8

97

17.2

CD105b

82

49.5

97.9

Sca 1b

89.9

67.5

53

CD29

99.2

99.4

96.2

CD44

95.1

98.7

99.7

CD45b

0.6

4

0.6

CD34b

0.4

0.4

0.8

CD31b

0.4

0.5

1.3


aData represents mean ± standard deviation of triplicate assays; b % positive cells; GAG, glycosaminoglycan; MHC, major histocompatibility complex.

Sullivan et al. Arthritis Research & Therapy 2012 14:R167   doi:10.1186/ar3916

Open Data