Additional file 1.

Figure S1 Gating strategy. Mononuclear cells were incubated in 4 separate tubes containing the following monoclonal antibodies: a) Isotypen-specific control antibodies; b) IgD-FITC, CD27-APC, CD38-PE/Cy7, CD45-APC/H7, CD19-HorizonBlue, CD24-PE, CD3-/CD14-PerCP; c) IgD-FITC, CD27-APC, CD38-PE/Cy7, CD45-APC/H7, CD19-HorizonBlue, CD80-PE, CD3-/CD14-PerCP; d) IgD-FITC, CD27-APC, CD38-PE/Cy7, CD45-APC/H7, CD19-HorizonBlue, CD95-PE, CD3-/CD14-PerCP. CD45 positive leukocytes were gated and after eliminating PerCP positive T cells and monocytes, B cells were separated according to their CD27 and IgD expression. Thereafter the expression of CD38, CD80 or CD95 was analyzed in each B cell population.

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Brezinschek et al. Arthritis Research & Therapy 2012 14:R161   doi:10.1186/ar3901