Additional file 4.
Figure S4. Modulation of TGase activity is associated with TG2 regulation. (A-C) TGase activity was measured by using in situ 5-(biotinamido)-pentylamine incorporation assay in (A) C-FLSs or A-FLSs cultured on gelatin matrix. (B) A-FLSs were transfected with control- or TG2-shRNA-expressing lentivirus. (C) C-FLSs were incubated with TGF-β (20 ng/ml), DTT (1 mM), or cystamine (100 μΜ).
Format: PDF Size: 9.8MB Download file
This file can be viewed with: Adobe Acrobat Reader
Lauzier et al. Arthritis Research & Therapy 2012 14:R159 doi:10.1186/ar3899