Activation of HUVECs and induction of apoptosis. (A) Expression of E-selectin, intercellular adhesion molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) was analyzed with flow cytometry against HUVECs treated with 640 μg/ml of control and two anti-FLRT2-positive IgGs, B11-8 and X10-48. Representative graphs (left) and summarized graph (right) are shown. (B) HUVECs treated with control IgG and two anti-FLRT2-positive IgGs, B11-8 and X10-48, for 24 hours were stained with annexin V and 7-AAD and analyzed with flow cytometry. (Annexin V positive/7-AAD negative) cells were measured as apoptotic cells. Representative graphs (left) and summarized graph (right) are shown. Error bars indicate SD.
Shirai et al. Arthritis Research & Therapy 2012 14:R157 doi:10.1186/ar3897