Expression of Th17 transcription factors RORγt in T cells from uveitis patients with Behçet's disease. (A) By flow cytometric analysis, fresh CD4 T cells from an active uveitis patient with Behçet's disease (BD) (middle histogram), an inactive uveitis patient treated with infliximab (IFX-treated BD: right histogram), or a healthy donor (HD: left histogram) were stained with anti-RORγt Abs and anti-CD4 Abs. (B) Th17 cell lines from the active uveitis patient with BD were co-cultured with infliximab and then stained with anti-RORγt Abs. The numbers in the histograms indicate the percentages of cells that were double-positive for RORγt/CD4. Abs, antibodies; RORγt, retinoid-acid receptor-related orphan receptor gamma t.
Sugita et al. Arthritis Research & Therapy 2012 14:R99 doi:10.1186/ar3824