Capacity of infliximab to inhibit Th17 cells from uveitis patients with Behçet's disease. (A) For ELISA analysis, polarized Th17 cell lines from a Behçet's disease (BD) patient (black bars) were co-cultured with infliximab (IFX). As controls, T cells from a sarcoidosis patient (SAR, black bars) and a healthy donor (HD, open bars) were exposed to IFX. The graph indicates the amount of IL-17 determined by ELISA (pg/ml). *P < 0.05, **P < 0.005 between two groups. (B) The polarized Th17 cell lines from BD were co-cultured with recombinant human TNF-α (5, 50, and 500 ng/ml), and then the supernatants were harvested to evaluate IL-17 production by ELISA (pg/ml). **P < 0.005, compared with control data without rTNF-α (open bar). (C) For flow cytometric analysis, murine Th17 cell lines induced from a spleen of a normal C57BL/6 mouse were stained with anti-mouse IL-17 abs and anti-mouse CD4 Abs after permeabilization. The lower histogram indicates mouse Th17 cell lines co-cultured with rTNF-α. The numbers in the histograms indicate the percentages of cells that were double-positive for IL-17/CD4. Right panel: The mouse Th17 cell lines were co-cultured with recombinant mouse TNF-α (5, 50, and 500 ng/ml), and then the supernatants were harvested to evaluate IL-17 production by ELISA (ng/ml). *P < 0.05, **P < 0.005, ***P < 0.0005, compared with control data without rTNF-α (open bar). Abs, antibodies.
Sugita et al. Arthritis Research & Therapy 2012 14:R99 doi:10.1186/ar3824