In vitro effects of infliximab on CD4+ T cells from uveitis patients with Behçet's disease. CD4+ T cells from Behçet's disease (BD) patients (black bars) with active uveitis or healthy donors (HD, open bars) were co-cultured with rIL-2 and anti-human CD3/CD28 abs in the presence of infliximab (IFX). As the control abs for infliximab, anti-human TNF-α monoclonal antibody (αTNF-α) or anti-human IL-6 monoclonal antibody (αIL-6) were used. The levels of cytokines such as IFN-γ (A), IL-4 (B), IL-10 (C), TNF-α (D), IL-6 (E), and IL-17 (F) in supernatants of T cells were evaluated by ELISA or CBA. Bars, mean ± SEM. Cytokine production by T cells. Asterisks mean values significantly higher than medium only (Abs (-)): *P < 0.05, **P < 0.005, ***P < 0.0005. Abs, antibodies; CBA, cytometric beads array; n.s., not significant; SEM, standard error of the mean.
Sugita et al. Arthritis Research & Therapy 2012 14:R99 doi:10.1186/ar3824