Table 1

Primer sequences used in quantitative real-time polymerase chain reaction

Gene

Primer

Direction

Reference or GenBank accession number


Beta-actin

5'-AAGCCACCCCACTTCTCTCTAA-3'

5'-AATGCTATCACCTCCCCTGTGT-3'

Forward

Reverse

[36]

Aggrecan

5'-AGGGCGAGTGGAATGATGTT-3'

5'-GGTGGCTGTGCCCTTTTTAC-3'

Forward

Reverse

[36]

Collagen I

(COL1A2)

5'-TTGCCCAAAGTTGTCCTCTTCT-3'

5'-AGCTTCTGTGGAACCATGGAA-3'

Forward

Reverse

[36]

Collagen II

(COL2A1)

5'-CTGCAAAATAAAATCTCGGTGTTCT-3'

5'-GGGCATTTGACTCACACCAGT-3'

Forward

Reverse

[36]

Collagen × (COL10A1)

5'-CAAGGCACCATCTCCAGGAA-3'

5'-AAAGGGTATTTGTGGCAGCATATT-3'

Forward

Reverse

[35]

IHh

5'-CCTTGTCAGCCGTGAGGCCG-3'

5'-GCTGCCGGCTCCGTGTGATT-3'

Forward

Reverse

NM_00218.3

MMP-13

5'-TGGAAGGATGCCTTTTTTTCTC-3'

5'-CACCCTCCCCAAGTATCAATAGG-3'

Forward

Reverse

NM_002427

SOX9

5'-CTTTGGTTTGTGTTCGTGTTTTG-3'

5'-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3'

Forward

Reverse

[31]

Versican

5'-TGGAATGATGTTCCCTGCAA-3'

5'-AAGGTCTTGGCATTTTCTACAACAG-3'

Forward

Reverse

[35]


All primers were purchased from Invitrogen (Mississauga, ON, Canada). COL1A2, type II collagen α2 chain; COL2A1, type I collagen α1 chain; COL10A1, type × collagen α1 chain; IHh, Indian Hedgehog; MMP-13, matrix metalloproteinase 13; SOX9, Sry-related HMG box 9.

Saliken et al. Arthritis Research & Therapy 2012 14:R153   doi:10.1186/ar3889

Open Data