Table 1 |
|||
|
Primer sequences used in quantitative real-time polymerase chain reaction |
|||
|
Gene |
Primer |
Direction |
Reference or GenBank accession number |
|
|
|||
|
Beta-actin |
5'-AAGCCACCCCACTTCTCTCTAA-3' 5'-AATGCTATCACCTCCCCTGTGT-3' |
Forward Reverse |
[36] |
|
Aggrecan |
5'-AGGGCGAGTGGAATGATGTT-3' 5'-GGTGGCTGTGCCCTTTTTAC-3' |
Forward Reverse |
[36] |
|
Collagen I (COL1A2) |
5'-TTGCCCAAAGTTGTCCTCTTCT-3' 5'-AGCTTCTGTGGAACCATGGAA-3' |
Forward Reverse |
[36] |
|
Collagen II (COL2A1) |
5'-CTGCAAAATAAAATCTCGGTGTTCT-3' 5'-GGGCATTTGACTCACACCAGT-3' |
Forward Reverse |
[36] |
|
Collagen × (COL10A1) |
5'-CAAGGCACCATCTCCAGGAA-3' 5'-AAAGGGTATTTGTGGCAGCATATT-3' |
Forward Reverse |
[35] |
|
IHh |
5'-CCTTGTCAGCCGTGAGGCCG-3' 5'-GCTGCCGGCTCCGTGTGATT-3' |
Forward Reverse |
|
|
MMP-13 |
5'-TGGAAGGATGCCTTTTTTTCTC-3' 5'-CACCCTCCCCAAGTATCAATAGG-3' |
Forward Reverse |
|
|
SOX9 |
5'-CTTTGGTTTGTGTTCGTGTTTTG-3' 5'-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3' |
Forward Reverse |
[31] |
|
Versican |
5'-TGGAATGATGTTCCCTGCAA-3' 5'-AAGGTCTTGGCATTTTCTACAACAG-3' |
Forward Reverse |
[35] |
|
|
|||
|
All primers were purchased from Invitrogen (Mississauga, ON, Canada). COL1A2, type II collagen α2 chain; COL2A1, type I collagen α1 chain; COL10A1, type × collagen α1 chain; IHh, Indian Hedgehog; MMP-13, matrix metalloproteinase 13; SOX9, Sry-related HMG box 9. |
|||
|
Saliken et al. Arthritis Research & Therapy 2012 14:R153 doi:10.1186/ar3889 |
|||
