Open Access Research article

B-cell depletion therapy in patients with diffuse systemic sclerosis associates with a significant decrease in PDGFR expression and activation in spindle-like cells in the skin

Dimitrios Daoussis1*, Athanassios C Tsamandas2, Stamatis-Nick C Liossis1, Ioannis Antonopoulos1, Elli Karatza2, Georgios Yiannopoulos1 and Andrew P Andonopoulos1

Author Affiliations

1 Division of Rheumatology, Department of Internal Medicine, Patras University Hospital, University of Patras Medical School, Patras, Rion, 26504, Greece

2 Department of Pathology, Patras University Hospital, University of Patras Medical School, Patras, Rion, 26504, Greece

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Arthritis Research & Therapy 2012, 14:R145  doi:10.1186/ar3879

Published: 14 June 2012

Additional files

Additional file 1:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 1 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.5MB Download file

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Additional file 2:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 2 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.5MB Download file

Open Data

Additional file 3:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 3 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 4:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 4 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 5:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 5 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.3MB Download file

Open Data

Additional file 6:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 6 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 7:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 7 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 8:

Phosphorylated PDGFRβ expression in skin biopsies from Patient 8 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.5MB Download file

Open Data

Additional file 9:

Phosphorylated PDGFRβ expression in skin biopsies from control Patient 1. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 10:

Phosphorylated PDGFRβ expression in skin biopsies from control Patient 2. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 2MB Download file

Open Data

Additional file 11:

Phosphorylated PDGFRβ expression in skin biopsies from control Patient 3. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.

Format: TIFF Size: 1.4MB Download file

Open Data