Research article
B-cell depletion therapy in patients with diffuse systemic sclerosis associates with a significant decrease in PDGFR expression and activation in spindle-like cells in the skin
- † Equal contributors
1 Division of Rheumatology, Department of Internal Medicine, Patras University Hospital, University of Patras Medical School, Patras, Rion, 26504, Greece
2 Department of Pathology, Patras University Hospital, University of Patras Medical School, Patras, Rion, 26504, Greece
Arthritis Research & Therapy 2012, 14:R145 doi:10.1186/ar3879
Published: 14 June 2012Additional files
Additional file 1:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 1 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.5MB Download file
Additional file 2:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 2 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.5MB Download file
Additional file 3:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 3 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
Additional file 4:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 4 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
Additional file 5:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 5 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.3MB Download file
Additional file 6:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 6 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
Additional file 7:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 7 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
Additional file 8:
Phosphorylated PDGFRβ expression in skin biopsies from Patient 8 (rituximab treated). A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.5MB Download file
Additional file 9:
Phosphorylated PDGFRβ expression in skin biopsies from control Patient 1. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
Additional file 10:
Phosphorylated PDGFRβ expression in skin biopsies from control Patient 2. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 2MB Download file
Additional file 11:
Phosphorylated PDGFRβ expression in skin biopsies from control Patient 3. A and B, biopsy taken at baseline; C and D, biopsy taken at 6 months. B and D, higher magnification of the areas included in the boxes in A and C, respectively. Red arrows indicate presence of spindle-like cells that express phospho-PDGFRβ. Streptavidin-biotin peroxidase: A and C, ×100; B and D, ×400.
Format: TIFF Size: 1.4MB Download file
