Table 2

Osteoblast gene expression in primary and fourth passage (P4) SF-derived osteoblast cultures from patients with juvenile idiopathic arthritis*

Primary cultures

Gene

oJIA (n = 12)

pJIA (n = 4)

Day 14

Day 21

Day 14

Day 21

Runx2

0.02 (0.01-0.07)

0.05 (0.02-0.11)

0.00 (0.00-0.01)

0.02 (0.00-0.05)

P (vs. pJIA)

0.02

0.18

P (vs. day 21)

0.20

0.34

OPG

1.63 (0.39-5.65)

13.99 (8.22-21.82)

0.68 (0.40-1.48)

1.52 (0.50-5.68)

P (vs. pJIA)

0.47

0.03

P (vs. day 21)

0.001

0.49

RANKL

0.08 (0.00-0.16)

0.18 (0.04-0.50)

0.12 (0.03-0.20)

0.16 (0.06-0.20)

P (vs. pJIA)

0.54

0.90

P (vs. day 21)

0.42

0.89

P4 cultures

oJIA (n = 7)

pJIA (n = 4)

Day 10

Day 14

Day 10

Day 14

Runx2

0.04 (0.02-0.08)

0.03 (0.02-0.05)

0.02 (0.01-0.02)

0.02 (0.01-0.03)

P (vs. pJIA)

0.02

0.32

P (vs. day14)

0.46

0.20

OPG

0.07 (0.04-0.08)

0.12 (0.08-0.20)

0.04 (0.01-0.06)

0.04 (0.01-0.06)

P (vs. pJIA)

0.23

0.01

P (vs. day14)

0.04

0.89

RANKL

0.01 (0.01-0.03)

0.01 (0.01-0.04)

0.00 (0.00-0.00)

0.01 (0.00-0.01)

P (vs. pJIA)

0.04

0.23

P (vs. day14)

0.53

0.06

*Values are median (interquartile range), statistical differences were calculated using the Mann-Whitney test. Patients were subdivided into the oligoarticular (oJIA) or polyarticular (pJIA) group according to the International League of Associations for Rheumatology (ILAR) criteria [3]. Day, day of osteoblast culture; OPG, osteoprotegerin gene; RANKL, receptor activator of NF-κB ligand gene; Runx2, runt-related transcription factor 2 gene.

Lazić et al. Arthritis Research & Therapy 2012 14:R139   doi:10.1186/ar3872