Table 1 |
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|
Sensitivity and specificity of our new IP-qPCR method. |
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|
Autoantibody specificitya |
n |
Anti-Th/To by IP-qPCR |
Anti-U3RNP by IP-qPCR |
|
|
|||
|
Scleroderma |
|||
|
|
|||
|
Th/To |
22 |
100% |
4.5% (1/22) |
|
U3RNP |
12 |
0% |
100% |
|
Topo I |
20 |
0% |
0% |
|
RNAPIII |
18 |
0% |
0% |
|
ACA |
15 |
0% |
0% |
|
PM-Scl |
5 |
0% |
0% |
|
|
|||
|
Others |
|||
|
|
|||
|
TMG |
3 |
0% |
100% |
|
La |
12 |
0% |
42% (5/12) |
|
NHS |
15 |
0% |
0% |
|
|
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|
Results show the high sensitivity and specificity of the new assay, for the detection of anti-Th/To and -U3RNP antibodies, compared with silver staining as gold standard. aAntibody specificity validated by IP, urea-PAGE, and silver staining. ACA, anti-centromere antibodies; IP, immunoprecipitation; NHS, normal human serum; RNAPIII, RNA polymerase III; TMG, trimethylguanosine; topo I, topoisomerase I. |
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|
Ceribelli et al. Arthritis Research & Therapy 2012 14:R128 doi:10.1186/ar3858 |
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