Table 1

Sensitivity and specificity of our new IP-qPCR method.

Autoantibody specificitya

n

Anti-Th/To

by IP-qPCR

Anti-U3RNP

by IP-qPCR


Scleroderma


Th/To

22

100%

4.5% (1/22)

U3RNP

12

0%

100%

Topo I

20

0%

0%

RNAPIII

18

0%

0%

ACA

15

0%

0%

PM-Scl

5

0%

0%


Others


TMG

3

0%

100%

La

12

0%

42% (5/12)

NHS

15

0%

0%


Results show the high sensitivity and specificity of the new assay, for the detection of anti-Th/To and -U3RNP antibodies, compared with silver staining as gold standard. aAntibody specificity validated by IP, urea-PAGE, and silver staining. ACA, anti-centromere antibodies; IP, immunoprecipitation; NHS, normal human serum; RNAPIII, RNA polymerase III; TMG, trimethylguanosine; topo I, topoisomerase I.

Ceribelli et al. Arthritis Research & Therapy 2012 14:R128   doi:10.1186/ar3858

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