Open Access Research article

Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity

Yang Li1, Li-dan Zhao1, Lu-sha Tong1, Su-ning Qian1, Yan Ren1, Lei Zhang1, Xin Ding1, Yang Chen1, Yan-xia Wang1, Wen Zhang1, Xiao-feng Zeng1, Feng-chun Zhang1, Fu-lin Tang1, Xuan Zhang1*, De-nian Ba2, Wei He2, Xue-tao Cao2 and Peter E Lipsky3*

Author Affiliations

1 Department of Rheumatology & Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 41# Da-Mu-Cang-Hu-Tong Street, Beijing 100032, China

2 Department of Immunology, School of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, #5 Dong-Dan-San-Tiao, Beijing 100005, China

3 Formerly National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

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Arthritis Research & Therapy 2012, 14:R123  doi:10.1186/ar3853

Published: 23 May 2012

Additional files

Additional file 1:

Supplementary Table S1 presenting characteristics of the SLE patients (n = 161).

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Additional file 2:

Supplementary Figure S1 presenting fluorescence-activated cell sorting (FACS) plots specifically showing CD200 expression in CD11c-CD123high plasmacytoid DCs (pDC) (Gate 2) and CD11c+CD123- myeloid DCs (mDC) (Gate 3).

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Additional file 3:

Supplementary Figure S2 showing the serum CD200 level did not correlate with the Systemic Lupus Erythematosus Disease Activity Index score, anti-dsDNA, IFNα, IL-6, or B-cell activating factor belonging to the TNF family (BAFF) in SLE patients.

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Additional file 4:

Supplementary Figure S3 showing expression of CD200R1 in naïve T cells (CD4+CD45RA+) and memory T cells (CD4+CD45RO+) of SLE patients tended to decrease compared with HCs, although it did not reach statistical significance (P = 0.50 and 0.11, respectively). Naïve T cells had less CD200R1 expression than memory T cells both in HCs and SLE patients (P = 0.0003 and P < 0.001).

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Additional file 5:

Supplementary Figure S4 showing immunoblot analysis of the expression of DOK2 (left) and p-DOK2 (right) in CD4+ T cells. CD200Fc induced phosphorylation of DOK2 (lane 2 on right).

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Additional file 6:

Supplementary Table S2 showing the effect of CD200 signaling on the differentiation of T-helper cell subsets.

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Additional file 7:

Supplementary Figure S5 showing the proportion of CD4+CD25highFoxp3+ T cells in new-onset active untreated SLE patients was significantly lower than in HCs (median 1.42, interquartile range 0.75 to 2.43 vs. 2.79, 1.95 to 4.52; P = 0.014). All cells plotted were CD4+.

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