Figure 2.

MSU induces IL-1β synthesis from SAA-primed synovial fibroblasts. A. Synovial fibroblasts were pretreated or untreated with the indicated concentrations of SAA for 12 h. After pretreatment, the cells were stimulated with the indicated concentrations of MSU for 24 h and supernatants were analyzed for IL-1β production by ELISA. Values represent the mean ± SD of three independent experiments. B. Synovial fibroblasts were pretreated or untreated with the indicated concentrations of SAA for 12 h. After pretreatment, the cells were stimulated with the indicated concentrations of MSU for 24 h and supernatants were analyzed by immunoblot for the presence of mature IL-1β. Three experiments were performed using different synovial fibroblasts and a representative result is shown.