Table 1

Distribution of FRβ in normal rat tissues analyzed using flow cytometry or immunohistochemistry

Flow Cytometric Analysis

FRβ

CD11 b/c


TGC-elicited Peritoneal mϕ

87 ± 3.1%

95 ± 2.5%

Immunohistochemical Analysis

FRβ

CD68

Spleen

White pulp

-

++

Red pulp mϕ

+

+++

Lymph node

Cortex, tingible body mϕ

-

++

Paracortical mϕ

-

+

Medullary mϕ

-

+

Subcapsular sinus mϕ

-

+

Brain

-

+

Liver mϕ (Kupffer cells)

+

++

Lung

Perivascular/peribronchial mϕ

-

+

Alveolar mϕ

-

+

Kidney

Mesangial mϕ

-

+

Heart

Myocardial mϕ

-

+

Endocardial mϕ

-

+

Colon

Mucosa

Epithelial mϕ

-

+

Lamina propria mϕ

+

+

Muscularis mucosa mϕ

-

+

Submucosal mϕ

-

+

Muscularis externa mϕ

-

+


Frequency of cell staining (-, <100/10 fields), (+, 100-200/10 fields), and (++, 200-300/10 fields), (+++, >300/10 fields). Data was obtained from three normal rat tissues with similar results. FRβ, folate receptor β; TGC, thioglycollate.

Nagai et al. Arthritis Research & Therapy 2012 14:R106   doi:10.1186/ar3831

Open Data