Reactivity of an anti-rat FRβ mAb with FRβ-expressing cells. (A) Non-transfected B300-19 cells (left) and rat FRβ gene-transfected B300-19 cells. (right) were stained using an anti-rat FRβ (4A67) mAb (black pattern) or isotype-matched irrelevant mAb (white pattern). Stained cells were analyzed using flow cytometry. Data are representative of three separate experiments. (B) TGC-elicited macrophages were double-stained using an anti-FRβ mAb (4A67) and PE-conjugated CD11b/c mAbs (OX42) and analyzed using flow cytometry. Numbers represent the percentage of cells within designated gates. Data are representative of three separate experiments. FRβ, folate receptor β; mAb, monoclonal antibody; TGC, thioglycollate.
Nagai et al. Arthritis Research & Therapy 2012 14:R106 doi:10.1186/ar3831