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Open Access Research article

The effects of oxygen tension and antiaging factor Klotho on Wnt signaling in nucleus pulposus cells

Akihiko Hiyama12*, Fumiyuki Arai12, Daisuke Sakai12, Katsuya Yokoyama12 and Joji Mochida12

Author Affiliations

1 Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan

2 Research Center for Regenerative Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan

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Arthritis Research & Therapy 2012, 14:R105  doi:10.1186/ar3830

Published: 2 May 2012

Additional files

Additional file 1:

Nucleus pulposus cells were cotransfected with the Klotho reporter plasmid along with 500 ng of Dkk1, Dkk2, Dkk3, Dkk4, or the empty backbone vector, and the reporter activity was measured (left panel). Nucleus pulposus cells were cotransfected with the Klotho promoter plasmid along with increasing quantities (100 to 500 ng) of the Dkk1 expression plasmid or with the empty backbone vector, and the reporter activity was measured (right panel). The results were normalized for transfection efficiency and are expressed as a relative ratio of luciferase to pGL4.74 activities (denoted as relative activity). *P < 0.05 between groups. Error bars represent SDs. NS, not significant.

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