Figure 5.

Cytosolic IκB and nuclear NF-κB protein expression in steroid-sensitive patients after macrophage migration inhibitory factor. Shift in cytosolic IκB and nuclear NF-κB protein expression in peripheral blood mononuclear cells (PBMCs) from the steroid-sensitive (SS) group after treatment with exogenous migration inhibitory factor (MIF) in the absence or presence of anti-MIF mAb. (A), (B) PBMCs from the SS group were first treated with exogenous MIF at different concentrations (0, 10, 25, 50 and 100 ng/ml) for 2 hours with or without previous treatment with anti-MIF mAb and then stimulated with dexamethasone at 0.42 mg/ml for 72 hours. NA, treatment only with exogenous MIF; MIF-MAb, treatment with exogenous MIF and anti-MIF mAb. Cytosolic IκB levels were determined by western blotting and quantified with installed density-analysis software. Electrophoretic mobility shift assay was used to assess the expression of nuclear NF-κB. Quantity One 4.4 software (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA)analyzed the results.