Figure 3.

Adiponectin mRNA regulation. (a) Confluent human skin fibroblasts (SFs) or subcutaneous preadipocytes (PAs) were incubated with 10 μM pioglitazone (Pio) for 48 hours, and RNA was examined by real-time quantitative polymerase chain reaction. The results, normalized with glyceraldehyde-3-phosphate dehydrogenase mRNA levels, represent the mean ± standard deviation of triplicate determinations. (b) Adiponectin mRNA levels in systemic sclerosis skin biopsies. The data were extracted from published genome-wide expression microarray datasets [16]. Levels were correlated with modified Rodnan skin score (MRSS).