The Sp1 transcription factor is essential for the expression of gliostatin/thymidine phosphorylase in rheumatoid fibroblast-like synoviocytes
1 Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, MuzuhoMizuho-ku, Nagoya, 467-8601, Japan
2 Department of Molecular Neurobiology, Nagoya City University Graduate School of Medical Sciences, Mizuho-Ku, 1 Kawasumi, Mizuho-cho, MuzuhoMizuho-ku, Nagoya, 467-8601, Japan
Arthritis Research & Therapy 2012, 14:R87 doi:10.1186/ar3811Published: 25 April 2012
Additional file 1:
Effect of cycloheximide on TNF-α induced GLS mRNA production in FLSs. Confluent FLSs in 6-well plate were incubated in the presence or absence of 10-100 (μg/ml cycloheximide for 30 min, followed by further incubation with TNF-α (1 ng/ml) for 24 h. The GLS mRNA levels are expressed as a RT-PCR product ratio (GLS/β-actin). Results are presented as mean ± SEM of four determinations. Statistical significance compared with controls was calculated using the Mann-Whitney U-test: compared to controls * P < 0.01; compared to samples with TNF-α alone † P < 0.01.
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