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Open Access Research article

The Sp1 transcription factor is essential for the expression of gliostatin/thymidine phosphorylase in rheumatoid fibroblast-like synoviocytes

Kenji Ikuta1, Yuko Waguri-Nagaya1*, Kae Kikuchi1, Takaya Yamagami1, Masahiro Nozaki1, Mineyoshi Aoyama2, Kiyofumi Asai2 and Takanobu Otsuka1

Author Affiliations

1 Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, MuzuhoMizuho-ku, Nagoya, 467-8601, Japan

2 Department of Molecular Neurobiology, Nagoya City University Graduate School of Medical Sciences, Mizuho-Ku, 1 Kawasumi, Mizuho-cho, MuzuhoMizuho-ku, Nagoya, 467-8601, Japan

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Arthritis Research & Therapy 2012, 14:R87  doi:10.1186/ar3811

Published: 25 April 2012

Additional files

Additional file 1:

Effect of cycloheximide on TNF-α induced GLS mRNA production in FLSs. Confluent FLSs in 6-well plate were incubated in the presence or absence of 10-100 (μg/ml cycloheximide for 30 min, followed by further incubation with TNF-α (1 ng/ml) for 24 h. The GLS mRNA levels are expressed as a RT-PCR product ratio (GLS/β-actin). Results are presented as mean ± SEM of four determinations. Statistical significance compared with controls was calculated using the Mann-Whitney U-test: compared to controls * P < 0.01; compared to samples with TNF-α alone † P < 0.01.

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