Table 1

Primer sequences used in real-time RT-PCR

Gene

Forward primer

Reverse primer

Mouse

Hprt

TCA GTC AAC GGG GGA CAT AAA

GGG GCT GTA CTG CTT AAC CAG

Prg4

GAA AAT ACT TCC CGT CTG CTT GT

ACT CCA TGT AGT GCT GAC AGT TA

Papss2

CCC GTG ATG GAG TCA TCA ACA TGA G

GTG CTT TGC AGT GGC TGT TCC

Plod2

CAC TGG ATT ATC CCA AAG AAG CCC

CCT GGC TTC TGC TTG ACT TAG GTT

Fxyd2

ATC CCT TCG AGT ACG ACT ATG AA

CAG CGG AAC CTT TTG CTG AGA

Bovine

Gapdh

GGG TCA TCA TCT CTG CAC CT

GGT CAT AAG TCC CTC CAC GA

Prg4

TGC CCT GAC TTC AAG AAG GAA TGC

CCA TAA TCG GAA CAG CAC TTG CCA

Papss2

TGC CAT CTT CCC ATC TCC CAT GTT

ACA GGT CTC TCT TGG TCT CAG GAT

Plod2

CCC TTT CCT ACC TCG ATT TCT GAA CAC

CCA TGT TTC TGG CTT CAG CTT GAC

Ramaswamy et al. Arthritis Research & Therapy 2012 14:R49   doi:10.1186/ar3762