TFM-C supresses the activation of macrophages. B6 mice recieved 10 μg/g TFM-C, celecoxib or vehicle on Day 0 and Day 2, and on Day 3, splenic macrophages were collected and were stimulated by LPS in vitro in the presence of TFM-C, celecoxib or vehicle. Cytokines were detected by ELISA. IL-1β and IL-6 were measured 24 h after stimulation. TNF-α was measured six hours after stimulation. The data shown are from a single experiment representative of three similar experiments. * P < 0.05 compared with control group, * P < 0.05 compared with celecoxib-treated group.
Chiba et al. Arthritis Research & Therapy 2012 14:R9 doi:10.1186/ar3683