Figure 1.

Lack of endogenous B cell-derived IL-10 results in exacerbated CIA. A. Chimeric mice were generated that lacked IL-10 specifically on B cells. Mean clinical score for CIA and numbers of diseased paws are shown. Data are expressed as mean ± SEM (n = 20, cumulative data from four separate experiments with n = 5 per experimental group). Data were compared by statistical analysis using the Fisher test. * P < 0.05. B. On Day 45 following immunization, mice were bled and the serum levels of total IgG1 and IgG2a CII-specific IgG antibodies were measured by ELISA. Data are expressed as mean ± SEM (n = 20, cumulative data from four separate experiments with n = 5 per experimental group). Data were compared by statistical analysis using the unpaired t test. * P < 0.05. C. The gradation of arthritis was scored from 0 to 4 according to the intensity of lining layer hyperplasia, mononuclear cell infiltration and pannus formation. Data are expressed as mean ± SEM (n = 20, cumulative data from four separate experiments with n = 5 per experimental group). Representative histological sections are also shown.