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Open Access Research article

An in vivo investigation of the initiation and progression of subchondral cysts in a rodent model of secondary osteoarthritis

David D McErlain12, Veronica Ulici3, Mark Darling4, Joseph S Gati2, Vasek Pitelka3, Frank Beier3 and David W Holdsworth125*

Author Affiliations

1 Department of Medical Biophysics; Schulich School of Medicine and Dentistry, The University of Western Ontario, 1151 Richmond Street, London, ON, Canada. N6A 3K7

2 Imaging Research Laboratories, Robarts Research Institute; Schulich School of Medicine and Dentistry, The University of Western Ontario, 100 Perth Drive, London, ON, Canada. N6A 5K8

3 Department of Physiology & Pharmacology; Schulich School of Medicine and Dentistry, The University of Western Ontario, 1151 Richmond Street, London, ON, Canada. N6A 3K7

4 Department of Pathology; Schulich School of Medicine and Dentistry, The University of Western Ontario, 1151 Richmond Street, London, ON, Canada. N6A 3K7

5 Department of Surgery; Schulich School of Medicine and Dentistry, The University of Western Ontario, 1151 Richmond Street, London, ON, Canada. N6A 3K7

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Arthritis Research & Therapy 2012, 14:R26  doi:10.1186/ar3727

Published: 3 February 2012

Additional files

Additional file 1:

Coronal section of the OA rat knee stained with Picrosirius Red (5× magnification). Subchondral cysts (SBC) within the medial tibial plateau stained positively for the presence of collagen within the lesion itself (black arrows). This confirms that the composition of the focal areas of bone loss, observed in co-registered micro-CT and MRI images, have a different composition than the normal marrow (yellow arrow). SBC appeared adjacent to the joint surface and were characteristically found in the region of the knee with the most severe cartilage degradation.

Format: TIFF Size: 1.8MB Download file

Open Data

Additional file 2:

Hematoxylin-eosin stain of the operated (ACLX) and un-operated (LEFT) knee of the same rat, 12 weeks post-surgery (10× magnification). Note the disorganization of the trabecular bone seen in the ACLX (arrow) versus the LEFT knee that occurred immediately adjacent the subchondral bone cyst (SBC).

Format: TIFF Size: 2.7MB Download file

Open Data