Table 2

Identification of immune response profiles by means of principal components analysis*

Cytokine

CMV/EBV

SEA/SEB

CPG

PMA/ionomycin

PHA

CD3/CD28

Media Alone


PC-1

PC-2

PC-1

PC-2

PC-1

PC-2

PC-1

PC-2

PC-1

PC-2

PC-1

PC-2

PC-1

PC-2


IL-17

0.54

0.27

0.75

0.40

0.31

0.11

0.38

0.58

0.24

0.43

0.81

0.23

0.86

GM-CSF

0.46

0.52

0.76

0.35

0.45

0.13

0.73

0.36

0.34

0.20

0.89

0.87

IL-1β

0.25

0.74

0.68

0.36

0.95

-0.13

0.76

0.92

0.20

0.18

0.55

0.92

IL-2

0.93

0.10

-0.27

0.17

0.31

0.27

0.73

0.37

0.66

0.14

IL-4

0.78

0.13

0.77

0.85

0.86

0.39

0.77

0.47

0.26

0.90

IL-5

0.79

-0.12

0.30

0.79

0.77

0.29

0.25

0.79

0.31

0.32

0.33

0.71

IL-13

0.93

0.12

0.39

0.89

-0.20

0.24

0.13

0.18

0.29

0.87

0.45

0.31

0.34

IL-10

-0.17

0.38

0.18

0.81

0.59

0.41

0.56

0.50

0.56

0.27

IL-12

0.48

-0.16

-0.26

0.87

-0.13

0.96

0.25

0.34

0.85

IFN-γ

0.85

0.36

0.28

0.13

0.25

0.53

0.63

0.75

0.43

0.41

MCP-1

0.48

0.27

0.72

0.19

0.60

0.88

0.71

0.29

0.15

-0.31

0.94

MIP-1β

0.29

0.20

0.57

0.25

0.72

0.35

0.29

0.43

0.37

0.57

0.34

0.67

IL-6

-0.21

0.86

0.87

0.23

0.71

0.95

0.15

0.76

0.20

0.79

0.93

TNF-α

0.39

0.33

0.47

0.23

0.91

0.80

0.20

0.65

0.49

0.56

0.67

0.87

IL-8

-0.37

-0.81

-0.49

-0.77

0.20

-0.88

0.23

-0.70

-0.23

-0.25

-0.29

-0.19

0.56

IL-7

0.23

-0.15

0.45

0.40

0.22

0.11

0.27

G-CSF

-0.13

0.96

0.84

0.15

0.82

-0.14

0.93

-0.17

0.85

0.29

0.19

-0.12

0.86

-0.13


*Principal components analysis was used to identify profiles of ex vivo cytokine production by peripheral blood mononuclear cells in response to stimulation. Shown are the component loadings of each cytokine in the first and second principal components of each stimulus. Bold values indicate loadings > 0.5. CD3/CD28, anti-CD3/anti-CD28 monoclonal antibodies; CMV/EBV, cytomegalovirus/Epstein-Barr virus; CPG, CpG oligonucleotides; G-CSF, granulocyte colony stimulating factor; GM-CSF, granulocyte macrophage colony stimulating factor; IFN-γ, interferon gamma; IL, interleukin; MCP, monocyte chemoattractant protein; MIP, monocyte inflammatory protein; PC, principal component; PHA, phytohemagglutinin; PMA, phorbol myristate acetate; SEA/SEB, Staphylococcal enterotoxin A/Staphylococcal enterotoxin B; TNF, tumor necrosis factor

Davis et al. Arthritis Research & Therapy 2012 14:R24   doi:10.1186/ar3706

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