Figure 2.

Effects of extracellular nicotinamide phosphoribosyltransferase on type II collagen expression and synthesis. Normal chondrocytes were treated with 5 μg/ml extracellular nicotinamide phosphoribosyltransferase (eNAMPT) overnight followed by stimulation with or without 50 ng/ml insulin-like growth factor-1 (IGF-1) for an additional 24 hours. (A) Total RNA was isolated, and quantitative real-time PCR was performed to determine mRNA expression of collagen II using the TATA box-binding protein (TBP) as a control. mRNA expression is presented as the fold change relative to control. Data presented as mean ± standard deviation of three independent experiments. (B) Cell lysates were collected, and the collagen II protein levels (normalized to DNA) were analyzed by ELISA and presented as the fold change relative to control. Data presented as mean ± standard deviation of three independent experiments.

Yammani and Loeser Arthritis Research & Therapy 2012 14:R23   doi:10.1186/ar3705
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