Additional file 1.

Methotrexate (MTX) has no effect on expression of citrullinated proteins and PAD4 and PAD2 in SFMCs. Brown diaminobenzidine immunoperoxidase staining detects citrullinated proteins, as detected with F95 antibody, PAD2 as detected with ROI001 antibody, and PAD4 as detected with SN823 antibody. Original magnification, ×200. Graphs show results of manual counting in six different SFMC samples analyzed in duplicate, and results are expressed as fold decrease of the number of positive cells in the treated samples as compared with controls.

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Makrygiannakis et al. Arthritis Research & Therapy 2012 14:R20   doi:10.1186/ar3702